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　　2000年畢業(yè)于上海第二醫(yī)科大學，獲博士學位。2001年2月至2004年1月在美國University of Pennsylvania從事博士后研究;2004年2月起在美國Northwestern University從事博士后研究，于2005年1月起任助理教授(研究型);2009年至2013年受聘于美國Washington State University，先后為助理教授(研究型)和副教授(研究型)。現(xiàn)任中國科學院上海生命科學院生物化學與細胞生物學研究所研究員、研究組長。

　　減數(shù)分裂的誕生是生命進化史上的標志性事件，意味著生命有了新的繁衍方式——有性生殖。因此，減數(shù)分裂是生物有性生殖的根本基礎(chǔ)，也是保證物種繁衍、染色體數(shù)目穩(wěn)定和物種適應環(huán)境變化而不斷進化的基本前提。所以，減數(shù)分裂的調(diào)控機制是探索生命奧秘的關(guān)鍵科學問題。

　　從單細胞酵母到哺乳動物，減數(shù)分裂的關(guān)鍵生物學過程包括同源染色體配對聯(lián)會、重組交換與分離都高度保守，然而不同生物體之間調(diào)控減數(shù)分裂的分子機制卻顯著不同。如絕大多數(shù)調(diào)控酵母、果蠅、線蟲減數(shù)分裂的關(guān)鍵基因，在哺乳動物中并無同源基因存在。減數(shù)分裂啟動是有絲分裂細胞轉(zhuǎn)換為減數(shù)分裂細胞的關(guān)鍵步驟。就生命科學研究現(xiàn)狀而言，學術(shù)界對哺乳動物減數(shù)分裂啟動的調(diào)控機制幾乎一無所知。同時，減數(shù)分裂至今還難以在體外實現(xiàn)，而在體外重建體內(nèi)的減數(shù)分裂過程對闡明遺傳穩(wěn)定性、基因多樣性的起源和表觀遺傳重編程發(fā)生的程度具有重大意義，對遺傳疾病的防治、干預和男性不育的治療以及對物種改良、動物保種都具有重要價值。

　　本實驗室將針對“減數(shù)分裂如何啟動?”這一重大科學問題，綜合應用分子生物學、遺傳學、功能基因組學、蛋白組學、系統(tǒng)生物學等技術(shù)手段，致力于以下研究：

　　1. 減數(shù)分裂啟動和發(fā)展的調(diào)控機制：繪制哺乳動物減數(shù)分裂啟動和發(fā)展過程中RNA(mRNA和非編碼RNA)和蛋白質(zhì)動態(tài)變化和其修飾圖譜及其調(diào)控的酶系圖譜，揭示這種動態(tài)變化及其修飾發(fā)生的分子基礎(chǔ)。

　　2. 減數(shù)分裂啟動和發(fā)展的表觀遺傳調(diào)控機制：以 RNA甲基化、組蛋白修飾和染色質(zhì)重塑為重點，闡明哺乳動物減數(shù)分裂啟動和發(fā)展過程中的表觀遺傳變化及其機制。

　　3. 微環(huán)境對減數(shù)分裂啟動和發(fā)展的作用及其機制

　　4. 減數(shù)分裂體外重建技術(shù)： 建立包括人精原干細胞在內(nèi)的精原細胞培養(yǎng)、體外分化及減數(shù)分裂的技術(shù)平臺，實現(xiàn)在培養(yǎng)皿中培養(yǎng)獲取單倍體精子細胞。

1. Xinzhe Tang^#, Zetao Hu^#, Jian Ding, Meixia Wu, Pin Guan, Yawei Song, Yue Yin, Wei Wu, Jinbiao Ma, Ying Huang^*, Ming-Han Tong^*. In vitro reconstitution of meiotic DNA double-strand-break formation. Nature（2025.2.19）639 (8055):800-807.(Comments on Nature by Francisco Mendez Diaz and Kevin D. Corbett ;Highlighted by Cell Research).
2. Zhen Lin^#, Bowen Rong^#, RuituLyu^#*, Yuxuan Zheng, Yao Chen, JunyiYan, Meixia Wu, Xiaogang Gao, Fuchou Tang^*, Fei Lan^*, and Ming-Han Tong^*. SETD1B-mediated broad H3K4me3 controls proper temporal patterns of gene expression critical for spermatid development. Cell Research 2025(35):345–361; https://doi.org/10.1038/s41422-025-01080-0. (Cover Story;Highlighted by Cell Research).
3. Zhen Lin^#, Bowen Rong^#, Meixia Wu, Junyi Yan, Tong Hong, Linjun Hou, Xinzhe Tang, Qiang Liu, Xiaozhong Peng, Yao Chen^*, Fei Lan^*, Ming-Han Tong^*. The KMT2 complex protein ASH2L is required for meiotic prophase progression but dispensable for mitosis in differentiated spermatogonia. Development (2025) 152, dev204630. doi:10.1242/dev.204630.
4. Yuefang Liu, Zhen Lin, Junyi Yan, Xi Zhang,?Ming-Han Tong*. A Rad50-null mutation in mouse germ cells causes reduced DSB formation, abnormal DSB end resection and complete loss of germ cells.?Development. 2024 Apr 15;151(8):dev202312. doi: 10.1242/dev.202312
5. Kejia Zhang, Tianxin Zhang, Yujie Zhang, Jinyu Yuan, Xinzhe Tang, Chaobao Zhang, Qianqian Yin, Yonglian Zhang,?Ming-Han Tong*. DNA/RNA helicase DHX36 is required for late stages of spermatogenesis.?Journal of Molecular Cell Biology. doi: 10.1093/jmcb/mjac069. (2022), 14(11), mjac069
6. Ying-Hua Wang^#, Meng Yan^#, Xi Zhang^#, Xin-Yu Liu, Yi-Fu Ding, Chong-Ping Lai,?Ming-Han Tong*, Jin-Song Li*. Rescue of male infertility through correcting a genetic mutation causing meiotic arrest in spermatogonial stem cells.?Asian Journal of Andrology. (2021) 23, 590–599
7. Yao Chen, Ruitu Lyu, Bowen Rong, Yuxuan Zheng, Zhen Lin, Ruofei Dai, Xi Zhang, Nannan Xie, Siqing Wang, Fuchou Tang*, Fei Lan* and?Ming-Han Tong*. 2020 Refined spatial temporal epigenomic profiling reveals intrinsic connection between PRDM9-mediated H3K4me3 and the fate of double-stranded breaks.?Cell Research. 30(3) , 256-268 .
8. Yu Jiang, Hui-Ying Zhang, Zhen Lin, Ye-Zhang Zhu, Chao Yu, Qian-Qian Sha,?Ming-Han Tong*, Li Shen*, and Heng-Yu Fan*. 2020 CXXC finger protein 1-mediated histone H3 lysine-4 trimethylationis essential for proper meiotic crossover formation in mice.?Development. 147, doi:10.1242/dev. 183764 .
9. Zhen Lin,?Ming-Han Tong*. 2019 m6A mRNA modification regulates mammalian spermatogenesis,?BBA - Gene Regulatory Mechanisms. 1862(3), 403-411.
10. Yao Chen, YuxuanZheng, Yun Gao, Zhen Lin, Suming Yang, Tongtong Wang, Qiu Wang, NannanXie, RongHua, Mingxi Liu, JiahaoSha, Michael D. Griswold, Jinsong Li*, Fuchou Tang* and?Ming-Han Tong*. 2018 Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis.?Cell Research. 28, 879-896.?(Cover Story,?Comments on?Cell Research?by NavinB. Ramakrishna and AzimSurani; F1000 prime recommendation;?Highlighted by NSR, 2018 Sanofi-Cell Research Outstanding Paper Award).
11. Xiaoli Zuo, Bowen Rong, Li Li, Ruitu Lv, Fei Lan*, and Ming-Han Tong* 2018 The histone methyltransferase SETD2 is required for expression of acrosin-binding protein 1 and protamines and essential for spermiogenesis in mice. Journal of Biological Chemistry. 293(24) 9188-9197 PMID: 29716999?
12. Tongtong Wang, Qianqian Yin, Xuehao Ma, Ming-Han Tong*, Yuchuan Zhou* 2018 Ccdc87 is critical for sperm function and male fertility. Biol Reprod. PMID: 29733332
13. Zhen Lin,Phillip J Hsu,Xudong Xing,Jianhuo Fang,Zhike Lu,Qin Zou,Ke-Jia Zhang,Xiao Zhang,Yuchuan Zhou,Teng Zhang,Youcheng Zhang,Wanlu Song,Guifang Jia, Xuerui Yang*,Chuan He*,Ming-Han Tong* 2017 Mettl3-/Mettl14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis.Cell Research. 27(10): 1216-1230 PMID: 28914256 ?(Highlighted by NSR)
14. Yao Chen,Li Ma,Cathryn Hogarth,Gang Wei,Michael D. Griswold*Ming-Han Tong* 2016 Retinoid signaling controls spermatogonial differentiation by regulating expression of replication-dependent core histone genes. Development. 143(9): 1502-1511 PMID: 26965368 ?(Highlighted article)
15. Tong, Ming-han* Li, Jinsong* 2015 Similarity of epigenetic reprogramming in primordial germ cells between human and mouse. National Science Review.12, 2(4): 384 *Corresponding author
16. Ming-Han Tong* , Qi-En Yang, Jeffrey C. Davis, and Michael D. Griswold* 2013?Retinol dehydrogenase 10 is indispensible for spermatogenesis in juvenile males. ProcNatlAcadSci U S A. 110(2): 543-8 PMID: 23267101
17. Ming-Han Tong*, Debra Mitchell, Samantha McGowan and Michael D.Griswold* 2012 Two miRNAs Clusters, Mir-17-92 and Mir-106b-25, Are Involved in the Regulation of Spermatogonial Differentiation. Biol Reprod. 86(3): 72 1-10 PMID: 22116806 *?Corresponding author
18. Ming-Han Tong, Debra Mitchell, Ryan Evanoff and Michael D.Griswold 2011 Expression of Mirlet7 Family MicroRNAs in Response to Retinoic Acid-Induced Spermatogonial Differentiation in Mice. Biol Reprod. 85 (1): 189-97 PMID: 21430230
19. Tong MH, Jiang H, Liu P, Lawson JA, Brass LF, Song WC. 2005?Spontaneous fetal loss caused by placental thrombosis in estrogen sulfotransferase-deficient mice.?Nat Med. 11(2): 153-9 PMID: 15685171